21 Burst results for "Vilnius"

Applications and Impact of CRISPR/CAS9 in Bioprocessing

Cell Culture Dish Podcast

05:48 min | 4 months ago

Applications and Impact of CRISPR/CAS9 in Bioprocessing

"Today. I'm joined by fanling. Wong director of cell line development and protein sciences and zane starkey wolf director of corporate development from wishy biologics. I'm excited to be speaking with both of you. Today about crisper cast nine technology and its possibilities in the discovery and development biopharmaceuticals. We will also conduct a deep dive on its potential impact on bioprocessing and bio manufacturing. Welcome fanling and zane to the podcast. Zinke randy glad to be here. Thank you brandy before we get too far and because of our audience is quite diverse with regards to their experiences in life sciences. Fenland could you please provide some background on. Crisper cast nine as molecular biology. Gene editing tool. Yeah sure so christmas. Nineteen action system is actually adapted from a natural procure arctic defense mechanism to bacteria to simplify. What could spec assistant can do is took leave the face she and i was. It has been incorporated into the bacteria routine on so that to keep the fate from reproducing. Crisper is actually akron stands for clusters servers regularly interspace. Shot had a dramatic repeats and kissed by the most well well-researched variant of the class outcasts nucleus. Which has been used within the gene editing function. So i think the research community have actually adapted this mechanism to revolutionize how we perform the genetic modifications Not only in pro arctic. But or so. You can arctic sales since the system was first published and zane i know from our previous conversations that you were saying that crisper casts has an intriguing origin. Would you be able to elaborate. Yes interesting research can be found on crisper that dates back to the late. Eighty s Other work has been conducted throughout the first decade of the two thousand however it wasn't until two thousand twelve that two pivotal research papers were published in the journal. Science one by jennifer down nov uc berkeley and manual shopping chair of the university of vienna and then another pianist by doctors cross unanimous and sickness at vilnius university. All demonstrating the use of bacterial. Crisper cast nine as simple programmable. Gene editing jewel. But i know that the story doesn't stop there does it no. It certainly does not in less than a year in two thousand thirteen. The labs of dr fung jong and will chong of the burden student. Mit dr george. Church's lab at harvard reported success in adapting. Crisper cast nine for genome editing in your area cells and both mouse and human cells. And i know that we could really spend an entire podcast. Just on the history of crisper so i wanted to stay focused on the technology here. There's been a lot of excitement since discovery about this molecular biology tool. Can you explain why. Sure the remarkable functionality of this tool is that it allows scientists to target specific locations within the genetic code of an organism to cut out or replace a segment of dna due to the high specificity and exactness of utility. The applications have far reaching potential. And it has already become a much to walks game changer. In many fields of life science because it enables efficient cost effective and precision gene. Editing that has a wide utility for development of biological therapeutics including so and gene therapy disease modeling diagnostics agriculture industrial biology and more. And this has me thinking just about alternatives to crisper casts altogether Are there other ways to edit genomes. And if so what makes a crisper cast so much better. Great question brandy many of the other gene editing systems utilize today such as zinc finger. Nicholas's talons the use of mega nick. Liaises or other by all vectors like a. Iv compared with christopher cast nine are in the end very complex and time intensive often requiring many more steps and thus are more costly as well also and this may be greatest benefit. Is that crisper chess. Nine as a low off target affects profile which again makes it an ideal gene. Editing tool justify along with that. I've read many Recent advances using crisper technologies. Could you elaborate a little bit on those. The advances are extensive and continuous. We speak one example includes crisper a crisper i which are techniques to up and down regulate gene expression using dead cast nine dead cast nine removed the nucleus capability of cast nine but still allows for the targeted binding to a double stranded. Dna sequence of interest using the highway. Specific guide are a that is one of the cornerstones of crisper genome editing. I'd like to add that another application. It's a using crisper for hamas directed repel or so called a the are so this technique in simple terms can repel a double stranded. Dna break which is very important for genus ability. But what does the crisper made. Sdr can do is that. It cannot only to repel a break. But or so crew. Eight the break and then replace it with a small mutation or as elijah sequences so this techniques have actually substantially opened ability or researchers to make gino added more quickly and more efficiently

Cell Line Development And Prot Zane Starkey Zinke Randy Arctic Dr Fung Jong Dr George Wong Vilnius University University Of Vienna Zane The Journal Berkeley Jennifer Harvard Nicholas Christopher Chess Hamas Elijah Gino
Belarus opens nuclear plant opposed by neighboring Lithuania

Weekend Edition Saturday

00:32 sec | 6 months ago

Belarus opens nuclear plant opposed by neighboring Lithuania

"The first nuclear power plant in Belarus has opened over the objections of neighboring Lithuania. The plant is about 25 miles away from Vilnius, the capital of Lithuania. President Alexander Lukashenko of Beller says the Russian built and finance plant quote will serve as an impetus for attracting the most advanced technologies Lithuanians, authorities have handed out free iodine pills to residents living near the Belarus border in case of a leak. Russia's state nuclear corporation insists it conforms to the highest

Lithuania President Alexander Lukashenko Belarus Vilnius Beller Russia
"vilnius" Discussed on Podcast RadioViajera

Podcast RadioViajera

06:07 min | 1 year ago

"vilnius" Discussed on Podcast RadioViajera

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"vilnius" Discussed on Podcast RadioViajera

Podcast RadioViajera

09:03 min | 1 year ago

"vilnius" Discussed on Podcast RadioViajera

"Few that allow money now shook US Chris. Three Co in better. Sarah could you share colonial in Ghana's in Santa yes you must meal Colorado Supreme Mutual. There's the Rosamund Pastel marching choosing got the at Las Colinas. There's the Alaska City few. He Common Oreo cookies than those in. The worker said on the term on the phillies or even the literature pairs of two theon guest anonymous androgynous director us. As you conduct quality process in the is the news ninety Never Komo's careers and Qaddafi. Yes in brief us. He must give it indicated. Lagaras police officer St Pecos. He umbrella Ruta dealers prices wealthy. The paper could Alberta Pedro Croquet Minimus Temple Castle Israeli car. Espn picking him on gun. To- yelling received POCO CON Guilma Finale. Twenty SHILPA travel the Paseo repetition three years younger employees. You're Super Beta Kappa. Saturday said get on Capitol. I'm Keila than the suppose dealers. Pass CEOS indicated few question important. This give it by a thorough like at the news is combined you like relish and Estanislao is allow much important toilet Lebron Mira kc city Kelly Missing Lugar in NC locate thoroughfare in into civilized another as momentum panel like relative freedom players. Ramallah journeys Davis who spoke to all the neoclassical wilty microphone finality to swing years anthem. Ups The stackable if he might throw lack appears and casino on this Thursday through script us and there are those years nobody Ducasse in is important this the daily Grand Duke really to Aena Be Gandhi L. governmental mass put Russia. They lead to one in this book. She programas in like after the rally throw neurological your Focus Quantico. Es them using Compagno conal think when they hit Islamic Doric am and do he goes to the engineers by not is anti war mutant female reaping thunder within Cuando Blanca Innovate Peter's Mukudi so parasail etc steelers to all throw Luger. Impressed indeed is directly me as to Las Colinas? The one time at rush team you as much important pace laterally. Mia's except looking at as Cook accuweather for forecast nine county ownership must partriotic. Course Lew Alto lateral you to Macquarie's us they let you pull these lateral Moselle. He put it should be star reverse. Total is killer care. Bala mutual winter is some is Li- Chea Della Sometime Arabia's rallied dildo course to TACO's is they for pre-med employer Alicia Talk. Sally Tonia India. The had we thoroughly here shows Alberta Winter. Moselle say let's you like Lhasa. The galleries been odious as compiled by the Qantara. Infant NBA's countries politicos asked if I am Plano and bless you lorraine doses to quite rather is more on Quadra Kenya's moines more important. They real news a seven Sheila if he sees you smell the robot. They listed Israel Amazon early one year. E Fonda in Alanya meakin interstate in when Tacoma's thin community streeters. Let's it you don't see. Other onto the steelers he Lutheran lemnos. Not Obviously today's premier. No alita locomotive. What me wash gather yet misgivings and Delta's zone. Pollock context to study Sean. Does this guitar is a match. Important is the Dylan Wa Polaka. They try to miscarry moral point and Paula. Cole Peters Join. In billionaire law suspicion we will need to see it the hippocampus. He only got a local resource seeing the India Motor Lemnos the faculties dilemma. Soon breath then. Blooming like three and see looking. F- for Sports I see giving almost colonial is by Maasai studios this story nationally. Lsu that he needs to milliliter in law. Terry naturally name is for what it does. How does concrete legless? Here's Donna Stimulus Devos economic news. Yes our MEISTER. They'll go Dakota view. Is that question leader? Comas later integrity. Raleigh stint though he quintaglie in Qena Polio. And they just feel like you. Should Your Buddies Ongoing Marco Contemplate La Porta smoothie kilter collect until the going to import British Mancuso partition format? Move Particulary Amazon. Which I think will so you must press. There are no he few someone brought colonial. Gamla very important if you're the classified material it's electoral politics. Kills if he says she will be a commodity and supposed be news it does. The S Allison is more moindre Sunday mass. One way of our Alberto Mama's boy meal. The latter knows muscle very hoops. Electoral Berea is Orelon. Was here yesterday. Steady to- cater his edict letters. Independent Film Really Miller industrial initiated the mushoo appropriate Constitucion Goma say commando BC NAMA SANDRA as multiplicity amass is turn granddaughters Pecos in star. A Punta. Puto lack of theon. They'll fouquet much mukuria shop a Komo Mandela on the return commodity theoretical. Plan your the rich uncles Shane gutters parachute is more curious book. Lessenthien Dario Roy Mirrors and yes WANNA has asthma perspicuous as he got us to St the EV. This in to escape on marginal passer so when we look at these meals. Hillary Laurent talk in the Alabama scarlata renews. What I could yourself is like. I gotTA story is allergists Clitoris Cape. What the underlying collateral. They'll forma picking a the Arctic avars to securities more cruisers. Komo tita rather in Kerala is just beginning Numero reference yet and Little Omar..

Las Colinas steelers Amazon Never Komo Alberta Pedro Croquet Minimus phillies Alaska Ghana Ramallah Colorado Supreme Mutual lateral Moselle Sally Tonia India Sarah Li- Chea Della Sometime Arabia Moselle Qaddafi Qantara Espn Kelly Missing Lugar SHILPA
"vilnius" Discussed on Skimm This

Skimm This

01:36 min | 1 year ago

"vilnius" Discussed on Skimm This

"Before we go today. We've got a fun fact coming to you from an airport port. In Lithuania security staff at Vilnius Airport are taking holiday spirit to a new level. They created a Christmas tree. Made up of banned items is reportedly confiscated from passengers over the last year. Think scissors peanut green to look like leaves and multi tools and lighters hung up as ornaments complete with a gold star at the top made of blades. That sort of look like cheese knives. The staff hopes that the tree reminds people of what not to pack and their carry ONS and how to stay off any airports reports naughty list. And that's all for Skim this thanks for listening and be sure to hit subscribe and rate and review US online. Line if you want to add to your morning routine sign up bar free newsletter. The daily skipped right on our website at the skin dot com. It's everything you need to know to start your day right in your inbox. The hello your friends from Skim. HQ here it's the most wonderful time of the year. And also also the busiest we asked and skimmers told us. They spend an average of twenty hours every winter. prepping for the holidays so we found a way to save you all awesome time. Introducing celebrates smarter shop with our presenting sponsor. Batiste we've skimmed the holidays and curated all the best products for you to give post up your travel game and just distress smarter shop online today at shop dot the Skim Dot Com. That's shop dot the skin dot com start shopping..

Vilnius Airport US Batiste Lithuania
ECB announces more stimulus and less growth

Bloomberg Daybreak: Europe

02:09 min | 2 years ago

ECB announces more stimulus and less growth

"Now, starting with an ECB preview central banks takes takes center stage, this morning that descends upon Vilnius Lithuania for his rate decision today's Caroline, just said Bloomberg's peo- skill. Most ski has the preview. The expected to offer generous terms on loans to banks when policymakers. And now they're this after the meeting in Vilnius prison. Mario drug Ethan, like escape questions about slowing inflation and the impact that is going to think pensions could have on the year area Konami with any cutting rate and opening the possibility to follow suit. The could find himself under pressure to deliver more stimulus down the line in Frankfurt gives cutting Muskie, Bloomberg daybreak Europe. So let's just say well Cranfield for my markets. Live team joins us central banks as we say taking center stage, the decision. Will it bring an end to the valley? Very good question. I'm sure a lot of people would like to know about it. Probably depends how the equity market in your decision today. What people are expecting as was mentioned. There is size stimulus of mareo druggy coming towards the end of his term. So he might not get many more chances to help out in terms of supporting European economies, which have been underperforming. So it's expected to offer some fresh long-term loans at decent rates for people that may help to stimulate things, but really the equity market reaction that would probably determine where we go. So if you're a paean stocks take it. Well, I live, so they can get a bit attraction than probably the, the rally we've seen in European bonds will probably take hold. But if the equity market is a bit more. So fit shrugs the decision than the ready will probably continue. So really how other asset classes perform moti Termine, but certainly, a lot of jitters around because also the Europeans are watching. What's going on, of course, in America, as well and trade talks on various fronts. So there's quite a few moving parts of the moment, right in as the Italians kind of puff up their chests, and, you know, double dare the EU to really do something about budgets. It's, it's really a fascinating game

Vilnius Bloomberg Lithuania Moti Termine EU Ethan Konami Caroline Frankfurt Mario Europe Muskie America
Ukraine, President And Nina Dunk discussed on Monocle 24: The Globalist

Monocle 24: The Globalist

01:04 min | 2 years ago

Ukraine, President And Nina Dunk discussed on Monocle 24: The Globalist

"Vladamir Zelinski the comedian actor who was elected president of Ukraine last month was inaugurated yesterday. He dissolved parliament and went on to give a speech about the way forward with a mole on joined by Nina dunk of its who is global fellow at the Wilson center, an expert on disinformation, and the former in the former communist space. She joins us from Vilnius Nina. We know that Celeski chose his spokesperson through social media will know that he's dissolved parliament will they be a snap election in the traditional way or always to expect something entirely different. Well, I'm not even one hundred percent short there will be a snap election, actually, because based on the constitution of Ukraine, given that the parliamentary coalition collapsed last week on Friday, technically the president is not allowed to dissolve parliament, although the preparations for an election in July seem to be going forward. And there was a lot of jubilation in the crowd gathered outside of Ukrainian parliament. Yesterday when this was announced, so we'll see. But certainly this presidency is off to a traditional start. I

Ukraine President Trump Nina Dunk Vilnius Nina Wilson Center Vladamir Zelinski Celeski One Hundred Percent
"vilnius" Discussed on PRI's The World

PRI's The World

04:13 min | 2 years ago

"vilnius" Discussed on PRI's The World

"Another way was the literal destruction of communities. The city of Vilnius in what's now. Lithuania is one case in point. Vilnius was once called the Jerusalem of the north because of the strength and vitality of its Jewish culture and learning and at the heart of Jewish Vilnius was the great synagogue are Schule in Yiddish the Nazis burnt down during World War Two. Then after the war, the Soviets level the ruins and built a school on top of it for three years. Now, archaeologists have been working on the site and the progress has been pretty remarkable. Richard Freund is part of the team Freund as a professor of Jewish history at the university of Hartford in Connecticut. I s m I about the historic significance Vilnius in Jewish culture. It was very dynamic location, and it was not only because of the religious life was because add cultural life. You had Zionists and you had secular Yiddish culture and literature and music. But the thing that was the epitome, the symbol of the story was this great synagogue and Schule Hof, which was a combination between a ninety. Second street y assisting chapel and the Vatican it was the size of a football field. And it was one of the great institutions of Europe in its time. You're the reference to the ninety. Second street y, we should underscore. It's not just a place to sleep like a hassle, but gathering space for all kinds of cultural events. Prior to World War Two, how old was a Jewish community and Vilnius and how old was a great synagogue? The Jewish community at Rives somewhere in the fifteenth century, and the first synagogues were established were were would. But the the final disposition of a great synagogue was probably in the seventeenth century. And so for about four hundred years, you have this institution that sitting in the middle of downtown Vilnius which was across. Roads of Europe and the development of the of the the culture was that not only was that a synagogue also had inside of the synagogue at had twelve smaller, synagogues and at every different cultural institution. And then it had a mass bath house in the back because the Jews and the Jewish quarter didn't have water in their homes. So they had to go at least three times a day to drink, go to the bathroom, take showers, and some people even went there to pray. Yes. So the ninety second street, y metaphor is really apt toes. What kind of progress you in the archaeologists are making? Well, you know, I'm a field archaeologist, that and we have a, the university of Hartford, has a geo physics and science team that goes out to sites. We've now done fifteen sites just in with the wanian forty, five sites worldwide. We go to a site and we can map the subsurface using ground penetrating radar and electrical relatively tomography, and no. No, what's there before we've excavate? And that's really what made this work so possible because the great synagogue was located underneath and elementary school downtown Vilnius. And but for this equipment, we wouldn't have been able to not only prove it, but then we were able to excavate in the play ground all of the artifacts. And once the the government saw just how many artifacts will coming out of this, they decided to close the school. Yeah, it sounds like the technology was crucial to not just as discovery but getting permission to do this research. And I think that this is the the one thing that is very important is the holocaust and genocide sites. When people think that everything is destroyed, actually may still be there below the surface. And this gives us an up between ity to go to the place. See if it's there. If it's burials, we can at least Mark them. So they're not desecrated again, but also you can excavate sites like the great sin. Agog we've already taken out hundreds of thousands of small fines and the great altar. The great Bima that was taken who has found this this summer really is remarkable as if we found Westminster Abbey buried underneath rubble. So the whole thing is that the great synagogue had secret..

Vilnius Jewish Vilnius university of Hartford Europe Lithuania Richard Freund Westminster Abbey Connecticut football professor four hundred years ninety second three years
"vilnius" Discussed on WNYC 93.9 FM

WNYC 93.9 FM

02:05 min | 3 years ago

"vilnius" Discussed on WNYC 93.9 FM

"I think i wrote a story about it yeah i went to lithuania vilnius lithuania to play a holocaust survivor a hungarian woman who was a doctor in the camps a jew who performed abortions in the camps to save the lives of the pregnant women because if the nazis got any word of any woman being pregnant they were immediately gasped because they didn't want babies around but i would my character would perform abortions and saved many lives and it was a really complicated character kind of a elitist and had you know had housekeepers in her in the camps i mean there was a whole system of hierarchy of of in that she cultivated in the camps but she was a really incredible woman and i watched every single documentary on the holocaust that i could get my hands on and read every single book this is just after nine eleven i'm i'm in vilnius i have three little kids at home in los angeles and i had a panic attack i the first one i've never had one since but i was in the day before we started shooting am the first day of shooting i'm i'm watching the smokes the smoke coming out of the stacks that the camp concentration camp and it's the smoke of the burning of my children so i'm already preparing myself emotionally for that and it's the we're in rehearsal and my heart just started to go and i thought this is i don't know what's i can't breathe and i they took me to the emergency room and this is lithuanian dr pointed to this open to this huge thick book and said these this is what you have said chronic anxiety disorder i know i'm like i'm not that person i'm just there's no way i'm having a heart attack check my heart she said no no this this is it so indeed i wasn't having hardly attack it with a panic attack and she gave me some what was it how what's the medicine for panic i don't know zanex.

lithuania vilnius los angeles
"vilnius" Discussed on WORT 89.9 FM

WORT 89.9 FM

02:35 min | 3 years ago

"vilnius" Discussed on WORT 89.9 FM

"I brought up national holocaust remembrance it's not a whole month it's just a day but it comes in april very fittingly i wanted to read this poem this entitled inside the pale of settlement in the pale of settlement when you hear the phrase beyond the pale that's beyond the pale people don't often realize that the derivation of that expression was that jews in the russian empire had to live within the pale of settlement so it's like saying oh that's beyond the ghetto jews were forced to live in in ghettos in various parts of the russian empire so this is entitled inside the pale of settlement and it takes place in vilnius lithuania on the baltic tour in this new millennium dr lindy's anger erupted in vilnius the guide stood with us outside the restored church rescued now from the days of godless communism inside it's all white marble snowdrift bridal veil and the crystal chandelier is a ship in full sail but on the sidewalk outside is a star embedded in the ground to mark the start of the line of linked hands that severed the links to communism our guide maria wants to tell us how with hands held and holding she became part of the resurrection of her beloved homeland lithuania but dr lindy wants maria to talk about the jews who wants people'd this city she wants jews to spring up from the re coupled stones she wants to see them walk through the university gates speaking of scripture but also of science art music she wants not just the sheva boys in black captains but the rebels who moved beyond the pale and the girls who would grasp a wider future then the double circumscription of jewish women in the russian empire she wants the kosher butcher shop the cabinet maker the.

lithuania dr lindy maria vilnius
"vilnius" Discussed on Cape Up with Jonathan Capehart

Cape Up with Jonathan Capehart

02:11 min | 3 years ago

"vilnius" Discussed on Cape Up with Jonathan Capehart

"Sure that i got back in town to be able to do this and i want to remind the audience into the room and those of you if i can find a camera those of you're watching by a livestream that you can pose questions via tweet by a twitter you can tweet your questions and comments to us using the hashtag post live so ms worry you i mean it is ten thirty yeah wait in here all morning is for for all of the panel so you've heard everything and this is the last discussion and i take it that you're not too happy with what you've been hearing or better yet not too happy about things that you haven't heard in some of the discussions what have you heard that you wish you had to vilnius since early morning and that also flew in from germany okay you whistle oh we're we're on the same wave this moment yeah i was very interesting the different panels were featured and speaking of different areas of the hiv aids epidemic in this was becoming again folks k bury our heads in the sand again as we had been for many years when i was appointed at your ambassador of health for the us um it was a selfimposed mandate that i made based on what i've is experiencing not personally but then again personally um i lost my valet who worked with me for many years to aids not knowing exactly what it was a third of his cancer like everybody else it from the very beginning of this epidemic thing um until rock cut super to face on it and gave it a name nobody knew what was and being as curious is i am it's my grandfather used to say my mother says nosy.

twitter vilnius hiv germany
"vilnius" Discussed on Filmhaus

Filmhaus

01:55 min | 3 years ago

"vilnius" Discussed on Filmhaus

"Um i absolutely i'll go does very specific instead of a grand idea but um i loved tux hoxha's is so good i loved huckster at this entire film and some people will point out parts of the vilnius dan like of you know huxley's moments i adored him and i think that ryan johnson figured out hawks ten times more edgy apron's figured out how expand not thrown room all there is no like fuck you i'm even when i love it haji good moment not throne room just the hit just a hand gesture yeah yeah just a hand gesture while it's everything it's just the curtains on the walls the royal guard that is smoke snow sits dick is so big i love it i love it okay well let's talk about all the stuff we hate because i got on my bullet points ready to go here petition or change dot org the ha i am later tonight petitioning ryan johnson himself a ryan johnson has to admit that last jedi is awful the golds petition is to make sure that the oh so mighty ryan johnson will know that we do not support his decisions make sure he knows what he created is not a masterpiece so there's a whole bullet list here uh i will get it at all to ultra i'll trickle amount because this i think represents everything that i believe firmly that my heart there you go all right now to do it now hours of oil and get there on time i'll just say right off the bat i did not think this was a good film and i thought it was a fund movie play as far as i always that star was pretty good at being a film and a good movie i thought it offered something that you could look at it and they were themes in a great pieces of it that like kind of trend transpires or any different things like it was just i don't know it was something always a much bigger in this when just felt like the cell almost worse than rogue one and a lot of ways but some people liked wrote to be clear i rector i didn't i didn't hate rogue one thing like is one of the things where like upon second viewing up okay maybe didn't hit it as much i was very surprises.

huxley ryan johnson hawks vilnius
"vilnius" Discussed on WSRQ Talk Radio

WSRQ Talk Radio

01:37 min | 3 years ago

"vilnius" Discussed on WSRQ Talk Radio

"Of the equipment that you should be using in how to use it and if you seemed to be running into trouble you stop and then you go to your professional and let them handle it and then in the meantime you can maintain it some maintenance is good but giving some equipment that they can handle for christmas given the stuff that's gonna that's going to help brushes and colmes and that kind of stuff their wonderful gifts to give people would pets yes if he messed up at home to take achieve groomer can think fixing the hit exactly but you know it's funny because uganda petsmart pedco you'll see professional grooming clippers now most people don't know what type of blade or what type of attached when goes onto these clippers if you use the wrong blade and i go into an area like a tuka poor ran an year i can remove the year who remove it on that in that study even a joke i can go into a talk of where i can go three foreign choosing before you realize that you've done something wrong and sometimes the dogs scream and sometimes they know it's a very very clean cuts or sometimes they don't feel it totes it's too you know it's already gone way too far key member years ago were that groomer did it and cut off the dogs here in superglued it in the vilnius didn't know until they got home in the ear fell off i do remember that yes i do remember that you know and again at that depth that's a case of just someone not knowing how to use the equipment you go in the wrong way you don't follow you know the lie of the code the way that the with the way the skin full folds in flab saw you're going to have a problem but they readily sell these these tools out there 'cause injury you know what and.

christmas clippers vilnius colmes uganda
"vilnius" Discussed on Fictional

Fictional

02:09 min | 3 years ago

"vilnius" Discussed on Fictional

"Reagan the darkness the three men who had attacked them but there's also another when that was far far ahead of the other three one that would get away bank will lay back on the hill and stared up at the sky his son flint's had escaped macbeth's knives his some would live and the prophecy be fulfilled oh exim finish up macbeth's and season won a fictional and hopefully i'll be schilling low better bright now it's time for the best of the worst the vilnius time is the blue snowman the boost demand while blue is not a snowman orpo even really man looking inside that blue metal suit is beer in a brilliant vertebra leads father dr brilliant was a scientist newman 1940s leave begley middle american town fairweather balloting his lifelong goal was to bring about blue snow a special snow that freezes anything it touches for reasons i'm not sure eld in the midwest knits which right now last thing anyone needs a snow that freezes anything it touches the gulf scientists with a dream and evil kids dr brilliant died young unlike the others he actually perfected his device he lifted to his daughter with one request that she used it to better the world somehow maybe the family was massively in debt because we're fathers longterm obsession with making snow worse or maybe she was just evil we don't know we do know was that she decided to not make the world a better place she was going to squeeze it for all the money that she could get but she didn't want to have to travel the cnet from her hilltop home she saw all the farmers a fair weather valley and she came up with their scheme her device could create blew snow blizzards but it could also create reverse blizzards she covered the fields of weather valium blue.

flint macbeth cnet Reagan schilling vilnius scientist newman gulf
"vilnius" Discussed on WPRO 630AM

WPRO 630AM

07:15 min | 3 years ago

"vilnius" Discussed on WPRO 630AM

"Man one inter you london be emme pentagon would she you added the get cooking the new cool although my knee good oh my oh my the middle my governor one my time work in vilnius alone right don't two my no congratulations way the oh cool care and the governor would have let go there you go the by the to get in i i will join us the m one may when they are not in june no me the move i iranian the way it the neither.

vilnius emme pentagon
"vilnius" Discussed on Orbital Paths

Orbital Paths

01:31 min | 3 years ago

"vilnius" Discussed on Orbital Paths

"Middle juices is actually many hundreds of lakers way so we wouldn't know that for hundreds of years as the shockwave got to us and what when bilges blows up it's not going to affect the earth but it will light up the night sky it'll be really spectacular you'll probably be able to read by it in the night sky for awhile it could happen tomorrow and you know i i'm always standing outside in the cold winter looking at beetlejuice you're looking at a star that's just about to die and then also in the belt stars the the lowest of belt stars the one farthest to the left won lowest down added that his are the stores in the bill in the vilnius ryan in the belt of orion the farthest one down on the left is actually called delta oreo onus and dealt or unice is one of the stars did my my doctoral dissertations on and i spent hours and hours towers hours observing the star through telescopes all over the world and use telescopes in arizona i use telescopes in australia um i actually used telescopes that were in space so i i mean i did lots of different observations of the star it took it would suspicions why did you pick this sir well it in their letter village was yet it's right it's right i i i did my dissertation on specifically very massive binary starts to it turns out that the stars actually two stars orbiting each other and they're so close together the human i can't separate them they the orbit so close together that the two stars gore roundabout but once every two.

lakers vilnius ryan arizona australia gore roundabout
"vilnius" Discussed on The Ryen Russillo Show

The Ryen Russillo Show

01:43 min | 3 years ago

"vilnius" Discussed on The Ryen Russillo Show

"Shark shark shark was one three defense and basically out so a little naive i wonder word knowing that of the three options that you're going with right exactly and then off i wanted to go to like to tracy porter interception that was we went to it was basically a blitz off the openside but we pressed are corner are getting a little too technical but ended deos one of those where we had always showed if who blitz off the openside that we are back or corners up which aims it up and made him a squad corner makes basically me they could jump so now he's sitting on the route and so he sat around manning because what you've done earlier henman into hitting him squint exactly and then he threw so you to the ceylon near the hero none at a university pours here yeah the orleans scored touchdown of course he's ever they look at the emily here a call her who cares zoo it's a team gabay what a town caught the ball with gordon because now we win he's the hero so after that we're here with vilnius will show you this being radio as it kind of is the precursor to what i want to do here with atlanta new england but yeah you didn't really have a super bowl hangover that's being said i can't say about belgium can brady i'm sorry i'm not maybe it's happening with atlanta it may have just happened with the cubs were feels like some of their players of regressed after winning a world series but you still won eleven and five what year you leave new orleans two thousand thirteen okay so venue the year after that a 30minute three in an 11 5 again you had to do in that lebanon fiveyear f you on the super bowl go up to play in eight in nine or seventy nine seattle team yes because they won the division in the new lost that playoff game to him yet did you feel like there was any kind of hang over at all or no no and i've always disagreed with that with that you don't believe the the hangover because.

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"vilnius" Discussed on Monocle 24: The Monocle Arts Review

Monocle 24: The Monocle Arts Review

01:54 min | 4 years ago

"vilnius" Discussed on Monocle 24: The Monocle Arts Review

"She would give her perspective on it and would be a perspective on the same event but my perspective on it is very different and we realized that over the course of promoting this film and talking about you know the the autobiographical nature of it the autobiography in the movie is is very subjective and it is my version of how i remember things and is very different from hers even though she was just as much part of it as i was so that's definitely all plays into it that's all part of it but i also don't ever consciously try to exploit those ideas are those themes they just sort of rear their head in spite of what i'm doing you'll film doesn't really asked people to believe in ghosts it doesn't ask people to suspend some sort of element of disbelief in order to enjoy the film it simply tells the story from the perspective all of someone who may home may not be a an actual real yang a from you'll perspective why do people believe in ghosts as a very comforting thought i mean it it is a way to hang on to those around us who who we've lost you know the idea that that someone we love who who we've lost might still be hanging around his comforting to us at the same time is also comforting thing that we might not have to leave that we might you know after after were gone we might be able to stick around ourselves and partake in the world that we understand so it's a way to to both hang on to things and to envision a world in which we might persevered past our own point of expiration and that's a very comforting concept the emotional aspect of you'll film and the sheer audacity i think of some of the themes in it as well that's something we never ever get to see in the cinema and i love a no load of the judges have remarked on this that you'll film i mean look at the poster it's a beautiful image it doesn't necessarily at will i mean probably can't tell you what to expect in this vilnius such an unexpected film in every pot all of its being.

vilnius
"vilnius" Discussed on Early to Rise Radio

Early to Rise Radio

01:53 min | 4 years ago

"vilnius" Discussed on Early to Rise Radio

"But in addition to that in addition to combat next year to vilnius enduring are ninth annual blacksmith event preparing for the tanna a big tent what else do you have planned for sovereign man freer agricultural fund for your trials for for the next year armies photos of the next year will be in james grow bank started mobile ago spent is incredibly exciting venture certain bankin so fame far there is a normal amount of regulation than for working through a long that but that will be dumping big every befo spine of next year a few other things of my sleep that are msa priority from just yet but then they will talk about those next year and will you how the back at the farm chile absolutely larisa the harvest season is a starts in early november so i expect to see you love for marine arms and how many how many acres of blueberries the government aga we will be five thousand total low and turkey of our moment humor is our number we grow who are general and that allows me to eat fourclass literacy day which is it that was my super bowl sunday cheat meal quarter points at blueberries all thank you so much sun y this was still a lot of fun doing a show i'm sure will do one again before next year maybe in after the camp for what we learn selects everybody for listening earlier rice radio this was definitely a unique show that you probably won't hear anywhere else i hope you enjoy o'clock is sick or by all right so i'd love to hear what you're going to do to start your journey to financial literacy.

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"vilnius" Discussed on No Jumper

No Jumper

01:43 min | 4 years ago

"vilnius" Discussed on No Jumper

"Highly anticipated i've been i've been damanour pomona sow for two three years now royals pulse will be the first south mud drop when i got when i was released from a pamphlet i end up doing gangland landscape blatter samoa weather but this one up top back the state bank ray or so you're thinking about doing the the first real album legacy when as you got albany decided to heated up i as soon as i got out i was on a oneoff tax mukhwana top act movement they probably one gonna be like the album nettie is now in but it was just a movement the the whole went up top akyama cns is the near movement it's like a culture is uh you know i mean we used to watch the movies eased to be tight one in nicaragua somebody any tactic gone back on emu filming in a just intensifying and showing me secere but when you really cut up in this kind of lifestyle as shit as she aid and i know cac in a bank vilnius is is is you gotta be ready when a pool alert so when i tap stay ready so you're in gotta get ready and so what's the untold again one up top which means one in the chamber on and it's a night rock act may brother right straight up so is just one at sabra straily hermoza to man you struck me as a guy who you might be the best gangsta rapper because you give of fuck about it more than anybody like eatery you know theor least rada yup good on it a bit donna forever opic cooking up says a kid in there i've been passionate about it um so you not say is serious i came up under a.

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"vilnius" Discussed on NewsRadio1620

NewsRadio1620

02:41 min | 4 years ago

"vilnius" Discussed on NewsRadio1620

"Thank you the vilnius manda this is the dave ramsey show rosie is in houston texas by rosie how will you hi how are you i quit my mom i should turn our china um you know like hurrying to turf skin on but shara what happened around two guys let infection although a he had had a stroke at learn you know faldo seventy seven here a on pardon we know there there's no will or you know out of occurring on a home in trying to figure out in order to her her whether any any bank accounts or anything like that her her bank account throwing has you know a bank for her own and in a car mmhmm okay retire near um i believe with hiring thing mmhmm oklahoma like you don't want it to the starter in on well the first thing you need to do how long ago to japan's london i'm sorry omer goodness we're like the the the business aspects of it or this in in the middle of you crying because your mom as well i agree with the kind of core a lot of support also knew a lot of people retired teacher let's go well what you need to get a get an attorney as the state law and other things out and you got to sit down with an attorney that does estate planning and they can teach you what you have to do it so i don't know texas law and on nonlawyer anyway so but most of the time in most states what amounts to is simply this you'll make a really good inventory of the things that she owns and the things that she does our assets on her liabilities and some of the assets that she have will have to pay her debts whatever debt she has what we paid out of our assets and as everything else has sold off then it'll likely be split between you and your brothers and sisters and like you at a sister you said show beef split between the two of you so that that list of assets list of liabilities list of thing she owns versus what she she does sit down without attorney bingo meet with him today if you want they can tell you.

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