Applications and Impact of CRISPR/CAS9 in Bioprocessing

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Today. I'm joined by fanling. Wong director of cell line development and protein sciences and zane starkey wolf director of corporate development from wishy biologics. I'm excited to be speaking with both of you. Today about crisper cast nine technology and its possibilities in the discovery and development biopharmaceuticals. We will also conduct a deep dive on its potential impact on bioprocessing and bio manufacturing. Welcome fanling and zane to the podcast. Zinke randy glad to be here. Thank you brandy before we get too far and because of our audience is quite diverse with regards to their experiences in life sciences. Fenland could you please provide some background on. Crisper cast nine as molecular biology. Gene editing tool. Yeah sure so christmas. Nineteen action system is actually adapted from a natural procure arctic defense mechanism to bacteria to simplify. What could spec assistant can do is took leave the face she and i was. It has been incorporated into the bacteria routine on so that to keep the fate from reproducing. Crisper is actually akron stands for clusters servers regularly interspace. Shot had a dramatic repeats and kissed by the most well well-researched variant of the class outcasts nucleus. Which has been used within the gene editing function. So i think the research community have actually adapted this mechanism to revolutionize how we perform the genetic modifications Not only in pro arctic. But or so. You can arctic sales since the system was first published and zane i know from our previous conversations that you were saying that crisper casts has an intriguing origin. Would you be able to elaborate. Yes interesting research can be found on crisper that dates back to the late. Eighty s Other work has been conducted throughout the first decade of the two thousand however it wasn't until two thousand twelve that two pivotal research papers were published in the journal. Science one by jennifer down nov uc berkeley and manual shopping chair of the university of vienna and then another pianist by doctors cross unanimous and sickness at vilnius university. All demonstrating the use of bacterial. Crisper cast nine as simple programmable. Gene editing jewel. But i know that the story doesn't stop there does it no. It certainly does not in less than a year in two thousand thirteen. The labs of dr fung jong and will chong of the burden student. Mit dr george. Church's lab at harvard reported success in adapting. Crisper cast nine for genome editing in your area cells and both mouse and human cells. And i know that we could really spend an entire podcast. Just on the history of crisper so i wanted to stay focused on the technology here. There's been a lot of excitement since discovery about this molecular biology tool. Can you explain why. Sure the remarkable functionality of this tool is that it allows scientists to target specific locations within the genetic code of an organism to cut out or replace a segment of dna due to the high specificity and exactness of utility. The applications have far reaching potential. And it has already become a much to walks game changer. In many fields of life science because it enables efficient cost effective and precision gene. Editing that has a wide utility for development of biological therapeutics including so and gene therapy disease modeling diagnostics agriculture industrial biology and more. And this has me thinking just about alternatives to crisper casts altogether Are there other ways to edit genomes. And if so what makes a crisper cast so much better. Great question brandy many of the other gene editing systems utilize today such as zinc finger. Nicholas's talons the use of mega nick. Liaises or other by all vectors like a. Iv compared with christopher cast nine are in the end very complex and time intensive often requiring many more steps and thus are more costly as well also and this may be greatest benefit. Is that crisper chess. Nine as a low off target affects profile which again makes it an ideal gene. Editing tool justify along with that. I've read many Recent advances using crisper technologies. Could you elaborate a little bit on those. The advances are extensive and continuous. We speak one example includes crisper a crisper i which are techniques to up and down regulate gene expression using dead cast nine dead cast nine removed the nucleus capability of cast nine but still allows for the targeted binding to a double stranded. Dna sequence of interest using the highway. Specific guide are a that is one of the cornerstones of crisper genome editing. I'd like to add that another application. It's a using crisper for hamas directed repel or so called a the are so this technique in simple terms can repel a double stranded. Dna break which is very important for genus ability. But what does the crisper made. Sdr can do is that. It cannot only to repel a break. But or so crew. Eight the break and then replace it with a small mutation or as elijah sequences so this techniques have actually substantially opened ability or researchers to make gino added more quickly and more efficiently

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